Htseq-count manual
HTSEQ-COUNT MANUAL >> READ ONLINE
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Given a file with aligned sequencing reads and a list of genomic features, a common task is to count how many reads map to each feature. A feature is here an Given mapped reads in a BAM file, this tool counts how many reads map to each gene using Ensembl gene These are illustrated in the HTSeq manual. 2 commonly used counting tools are featureCounts and htseq-count. The above tools only report the “raw” counts of reads that map to a single locationThese counts are performed using HTSeq2 and are calculated at the gene level. HTSeq-Count files are available in a tab-delimited format with one Ensembl The primary intended use case for htseq-count is differential expression analysis, where one compares the expression of the same gene across samples and not the
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